Study the Impact of Garlic Essential Oils Loaded Lipid Nanoparticles (GLNS) on Some Physiological Parameters in Male Rats

Abstract

The aims of work is detect the impact antioxidant garlic essential oils loaded lipid nanoparticles (GLNs) in male rats. Thirty adult male Wister rats weighing 148±10 g at 90 days of age were split into five groups.Group 1 were administered High-fat feed with 30% animal fat added was given for 60 days and Second group were control group , the Second group were administered garlic essential oil (GO) (10 mg\kg\day) for 28 days, fourth group administered with free LNPs (20mg \ Kg) and the fifth group administered with LNPs-GO (20mg\ kg \day). The result of X-Ray diffraction of LNPs-GO in major peak at 28.56° in the diffract to gram has moved to a lower 2θ value.The Zeta Potential (ZP) values of all formulations prepared were measured to be negative. The result indicates that the ZP ranges between 33.7 mV to -48.7 mV with the smallest size comprising the GLNs. The PDI value in the case of LNPs- GO were 0.265. regarding of Antioxidant Indicators the Blood Malondialdehyde Level (MDA) revealed that Glutathione Levels in Blood (GSH) significantly increased (P<0.05) in the G3, G4, and G5 groups relative to the G1 group, but they significantly decreased (P<0.05) in the G1 group.

The results also revealed that the G3 and G4 groups had significantly lower liver enzyme levels (AST, ALT, and ALP) than the G1 group, however the G5 groups did not significantly differ from the G1 group. Additionally, there was a noteworthy rise (P<0.05) in the G3 and G4.